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Fig. 2 | BMC Biotechnology

Fig. 2

From: Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Fig. 2

Establishing balanced expression strains for pro-peptide and MTG. a The clones of GS115 (pro) were screened by Dot blot (anti-His-HRP).1–4 were four repeated of clones. b The protein (pro-peptide) of 20 μl culture supernatant was separated by Tricine-SDS-PAGE and stained with Coomassie blue under non-reducing conditions. c The co-expression of pro-peptide and MTG from screened colonies with various doses of Zeocin were experimented by Western blot under non-reducing conditions. d The enzyme activity of MTG from screened clones with various doses of Zeocin were experimented. Three individual clones were tested for each condition.***Represents a statistically significant difference of P < 0.001 compared with other two antibiotic dosage groups

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