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Table 1 The t1/2 at 60 °C and midpoint of thermal unfolding of recombinant xylanases

From: Mutagenesis of N-terminal residues confer thermostability on a Penicillium janthinellum MA21601 xylanase

Xylanases

Mutations

t1/2 at 60 °C

Tm (oC)

ΔTm(oC)

PjxA

–

30 s

55.3 ± 0.4

–

PTxA

T10Y/N11H/N12D/Y15F/N30 L

58 s

58.4 ± 0.5

3.1 ± 0.9

PjxA-DB

T2C/T29C

33.8 min

72.7 ± 0.6

17.4 ± 1.0

PTxA-DB

T2C/T10Y/N11H/N12D/Y15F/N30 L/T29C

53.6 min

76.6 ± 0.6

21.3 ± 1.0

  1. The half-lives at 60 °C were determined as residual activity by incubating at different times at 60 °C. All protein concentrations were 0.05 mg/mL, and the midpoint of thermal unfolding (Tm) was determined by differential scanning calorimetry. The parallel experiments in 3 times
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BMC Biotechnology

ISSN: 1472-6750

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