Fig. 1
(a) Amino acid sequence comparison of the N-terminal sequence of PjxA and other xylanases. DNAMAN 6.0 software and NCBI (https://www.ncbi.nlm.nih.gov/) were used to compare the amino acid sequence of acid xylanase PjxA and other xylanases. PjxA: Recombinant xylanase of Penicillium janthinellum (MA21601); TfxA: Thermostable xylanase from Thermomonospora fusca; EvXyn11TS: Engineering hyperthermostable enzyme. (b) The structure of the mutant PTxA-DB based on PDB: 3wp3.1A, an additional disulfide bridge between Cys2-Cys29 displayed in purple; mutations of Leu30 and mutated region indicated in yellow. The prediction and homology modeling of the 3D structure of PjxA were performed by SWISS-MODEL (http://swissmodel.expasy.org/)