Fig. 3

a Cytotoxicity (target cell killing) calculated during the 72 h coincubation in function of the ASN concentration (0–500 nM). b Cytotoxicity (target cell killing) calculated during the three 24 h periods in presence (500 nM) or absence of ASN. c Schematic representation of the washout experiment (top). The grey W boxes indicate the ASN washouts. Cytotoxicity (target cell killing) calculated during the 48 h coincubation. All data are shown as the median with 95% confidence intervals of three independent experiments. N = 3. Significance is determined by a standard t-test, * = p ≤ 0.05,** = p ≤ 0.01