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Fig. 2 | BMC Biotechnology

Fig. 2

From: Modulation of chimeric antigen receptor surface expression by a small molecule switch

Fig. 2

a Proliferation of T-cells in the presence of increasing concentrations of Asunaprevir. The total number of cells at different days cultured in presence of 100 nM, 500 nM or 1000 nM relative to 0 nM ASN is presented. Data are shown as the median of PBMC from 2 donors done in duplicate. b Cytokine quantification after co-culture of anti-CD22 CAR T-cells with target cells as a function of Asunaprevir concentration. Data are normalized to the maximum value (with or without 500 nM ASN) and shown as the mean ± SD (duplicates). c Schematic representation of the experimental setup to determine the effect of ASN on SWIFF-CAR surface expression. d MFI of CAR positive cells 3 days post CAR transduction (day 7) in the absence (blue bars) or presence of 500 nM ASN. Data are shown as the mean ± SD (two T-cell donors). e MFI of CAR surface detection at day 10 of ASN dose response (0–1000 nM). Blue Dots: No ASN, “Red” dots: dose response of ASN. Green dots: washout of previously ASN (500 nM) treated T-cells. Data are shown as the mean ± SD (two T-cell donors)

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