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Table 4 Substrate specificity profile of SPPS enzyme from Pleurotus sajor-caju strain CTM10057

From: Purification and biochemical characterization of a novel thermostable protease from the oyster mushroom Pleurotus sajor-caju strain CTM10057 with industrial interest

Substrate Concentration Absorbance (nm) a Relative protease activity (%) b
Natural protein Casein 30 g/L 660 100 ± 2.5
Gelatin 30 g/L 660 80 ± 2.0
Keratin 30 g/L 660 37 ± 0.9
Ovalbumin 30 g/L 660 20 ± 0.5
BSA 30 g/L 660 10 ± 0.2
Modified protein Azo-casein 25 g/L 440 100 ± 2.5
Albumin azure 25 g/L 440 50 ± 1.8
Keratin azure 25 g/L 595 30 ± 1.2
Collagen type I 20 g/L 440 0 ± 0.0
Collagen type II 20 g/L 440 0 ± 0.0
Ester ATEE 10 mM 253 100 ± 2.5
BTEE 10 mM 253 96 ± 2.4
BAEE 10 mM 253 0 ± 0.0
BCEE 10 mM 253 0 ± 0.0
TAME        ↓ 10 mM 253 0 ± 0.0
Synthetic peptide (pNA)   Suc-F-pNA 5 mM 410 49 ± 1.5
Benz-Y-pNA 5 mM 410 0 ± 0.0
M-pNA 5 mM 410 0 ± 0.0
Ac-L-pNA 5 mM 410 0 ± 0.0
P-pNA 5 mM 410 0 ± 0.0
V-pNA 5 mM 410 0 ± 0.0
Ac-A-pNA 5 mM 410 62 ± 1.6
Benz-R-pNA 5 mM 410 0 ± 0.0
Suc-YLV-pNA 5 mM 410 0 ± 0.0
Suc-AAA-pNA 5 mM 410 50 ± 1.3
Suc-AAV-pNA 5 mM 410 64 ± 1.7
Suc-AAF-pNA 5 mM 410 62 ± 1.6
Benz-FVR-pNA 5 mM 410 0 ± 0.0
Suc-FAAF-pNA 5 mM 410 100 ± 2.5
Suc-AAPF-pNA 5 mM 410 97 ± 2.5
Suc-AAVA-pNA 5 mM 410 92 ± 2.4
Suc-AAPM-pNA 5 mM 410 77 ± 1.9
Suc-AAPL-pNA 5 mM 410 65 ± 1.7
Suc-LLVY-pNA 5 mM 410 57 ± 1.6
Ac-YVAD-pNA 5 mM 410 0 ± 0.0
  1. a Values represent means of three independent replicates, and ± standard errors are reported
  2. b The activity of each substrate was determined by measuring absorbance at specified wave lengths according to the relative method reported elsewhere [33, 34]
  3. BTEE, N-benzol-l-tyrosine ethyl ester; ATEE, N-acetyl-l-tyrosine ethyl ester monohydrate; BAEE, N-benzol-l-arginine ethyl ester; BCEE, S-benzyl-l-cysteine ethyl ester hydrochloride; TAME, Nα-p-tosyl- l-arginine methyl ester hydrochloride; N-succinyl-F-p-nitroanilide; N-benzoyl-Y-p-nitroanilide; M-p-nitroanilide; N-acetyl-L-p-nitroanilide; P-p-nitroanilide trifluoroacetate salt; V-p-nitroanilide hydrochloride; N-acetyl-A-p-nitroanilide; N-benzoyl-R-p-nitroanilide; N-succinyl-YLV-p-nitroanilide; N-succinyl-AAA-p-nitroanilide; N-succinyl-AAF-p-nitroanilide; N-succinyl-AAV-p-nitroanilide; N-benzoyl-FVR-p-nitroanilide; N-succinyl-FAAF-p-nitroanilide; N-succinyl-AAPF-p-nitroanilide; N-succinyl-AAVA-p-nitroanilide; N-succinyl-AAPM-p-nitroanilide; N-succinyl-AAPL-p-nitroanilide; N-succinyl-LLVY-p-nitroanilide; and N-acetyl-YVAD-p-nitroanilide