Skip to main content

Table 4 Substrate specificity profile of SPPS enzyme from Pleurotus sajor-caju strain CTM10057

From: Purification and biochemical characterization of a novel thermostable protease from the oyster mushroom Pleurotus sajor-caju strain CTM10057 with industrial interest

Substrate

Concentration

Absorbance (nm) a

Relative protease activity (%) b

Natural protein

Casein

30 g/L

660

100 ± 2.5

Gelatin

30 g/L

660

80 ± 2.0

Keratin

30 g/L

660

37 ± 0.9

Ovalbumin

30 g/L

660

20 ± 0.5

BSA

30 g/L

660

10 ± 0.2

Modified protein

Azo-casein

25 g/L

440

100 ± 2.5

Albumin azure

25 g/L

440

50 ± 1.8

Keratin azure

25 g/L

595

30 ± 1.2

Collagen type I

20 g/L

440

0 ± 0.0

Collagen type II

20 g/L

440

0 ± 0.0

Ester

ATEE

10 mM

253

100 ± 2.5

BTEE

10 mM

253

96 ± 2.4

BAEE

10 mM

253

0 ± 0.0

BCEE

10 mM

253

0 ± 0.0

TAME

       ↓

10 mM

253

0 ± 0.0

Synthetic peptide (pNA)

 

Suc-F-pNA

5 mM

410

49 ± 1.5

Benz-Y-pNA

5 mM

410

0 ± 0.0

M-pNA

5 mM

410

0 ± 0.0

Ac-L-pNA

5 mM

410

0 ± 0.0

P-pNA

5 mM

410

0 ± 0.0

V-pNA

5 mM

410

0 ± 0.0

Ac-A-pNA

5 mM

410

62 ± 1.6

Benz-R-pNA

5 mM

410

0 ± 0.0

Suc-YLV-pNA

5 mM

410

0 ± 0.0

Suc-AAA-pNA

5 mM

410

50 ± 1.3

Suc-AAV-pNA

5 mM

410

64 ± 1.7

Suc-AAF-pNA

5 mM

410

62 ± 1.6

Benz-FVR-pNA

5 mM

410

0 ± 0.0

Suc-FAAF-pNA

5 mM

410

100 ± 2.5

Suc-AAPF-pNA

5 mM

410

97 ± 2.5

Suc-AAVA-pNA

5 mM

410

92 ± 2.4

Suc-AAPM-pNA

5 mM

410

77 ± 1.9

Suc-AAPL-pNA

5 mM

410

65 ± 1.7

Suc-LLVY-pNA

5 mM

410

57 ± 1.6

Ac-YVAD-pNA

5 mM

410

0 ± 0.0

  1. a Values represent means of three independent replicates, and ± standard errors are reported
  2. b The activity of each substrate was determined by measuring absorbance at specified wave lengths according to the relative method reported elsewhere [33, 34]
  3. BTEE, N-benzol-l-tyrosine ethyl ester; ATEE, N-acetyl-l-tyrosine ethyl ester monohydrate; BAEE, N-benzol-l-arginine ethyl ester; BCEE, S-benzyl-l-cysteine ethyl ester hydrochloride; TAME, Nα-p-tosyl- l-arginine methyl ester hydrochloride; N-succinyl-F-p-nitroanilide; N-benzoyl-Y-p-nitroanilide; M-p-nitroanilide; N-acetyl-L-p-nitroanilide; P-p-nitroanilide trifluoroacetate salt; V-p-nitroanilide hydrochloride; N-acetyl-A-p-nitroanilide; N-benzoyl-R-p-nitroanilide; N-succinyl-YLV-p-nitroanilide; N-succinyl-AAA-p-nitroanilide; N-succinyl-AAF-p-nitroanilide; N-succinyl-AAV-p-nitroanilide; N-benzoyl-FVR-p-nitroanilide; N-succinyl-FAAF-p-nitroanilide; N-succinyl-AAPF-p-nitroanilide; N-succinyl-AAVA-p-nitroanilide; N-succinyl-AAPM-p-nitroanilide; N-succinyl-AAPL-p-nitroanilide; N-succinyl-LLVY-p-nitroanilide; and N-acetyl-YVAD-p-nitroanilide