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Table 1 Flow sheet purification of SPPS from Pleurotus sajor-caju strain CTM10057

From: Purification and biochemical characterization of a novel thermostable protease from the oyster mushroom Pleurotus sajor-caju strain CTM10057 with industrial interest

Purification step a Total activity (units) b × 104 Total protein (mg) b.c Specific activity (U/mg of protein) b Activity recovery rate (%) Purification factor (fold)
Crude extract 525 ± 13 590 ± 14 8823 100 1
Heat treatment (20 min at 80 °C) 430 ± 10 240 ± 6 17,917 82 2
(NH4)2SO4 fractionation (35–55%)-dialysis 375 ± 9 160 ± 4 23,437 71.4 3
FPLC (UNO Q-6) 152 ± 3 40 ± 1 38,000 28.5 4.3
HPLC (ZORBAX PSM 300 HPSEC) 79 ± 2 10 ± 0.5 79,000 15 9
  1. a Experiments were conducted three times and ± standard errors are reported
  2. b One unit (U) of protease activity was defined as the amount of enzyme required to release 1 μg tyrosine per minute under the experimental conditions used
  3. c Amounts of protein were estimated by the method of Bradford [30]