Fig. 4

Determination of the effects on the mtDNA sequence, changes in the expression of the p53 gene induced by UVR, and rescue by adding exogenous mitochondria using PAMM MitoCeption in the PBMC model. a, b mtDNA damage quantification by the 2^-ΔCT fold by qPCR after 3 min UVR exposure and rescue by PAMM Mitoception (n = 5, 5 PBMC donors exposed to UVR and PAMM of 3 donors). The qPCR 2^-ΔCT fold method was used. Primers: HB2M as the housekeeping gene sequence (Human B2M Beta-2-microglobulin, NCBI AH002619.1.), and HMito (designed for the mitochondrial genome, between positions 241 and 390, NCBI NC_012920.1) from Ajaz et al.(2015) [18] a 1 h culture after UVR exposure and PAMM MitoCeption. b 18 h culture after UVR exposure and PAMM MitoCeption. Analysis: Un-paired, ANOVA test and Student’s t-test are shown in the figure with an alpha-value of 0.05 (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) to establish statistically significant differences. c Analysis of the p53 gene expression after UVR damage and PAMM MitoCeption (n = 6, 6 PBMC donors exposed to UVR and PAMM of 3 donors). Primers for detecting p53 mRNA and for the nuclear PUM (housekeeping) were used to estimate p53 levels after UVR exposure and PAM MitoCeption in receptor cells with or without UVR damage. p53 gene expression was measured by qRT-PCR at 2 h after exposure and subsequent MitoCeption. Statistical analysis: Un-paired, ANOVA test and Student’s t-test are shown in the figure with an alpha-value of 0.05 (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) to establish statistically significant differences. Un-paired, ANOVA test and Student’s t-test (p < 0.05)