Fig. 4
From: Engineered Pichia pastoris production of fusaruside, a selective immunomodulator
LC-MS analysis of the sphingolipids from recombinant P. pastoris strains FUS2 (pPIC3.5 K − Δ3(E)-sd − Δ10(E)-sd), FUS3 (pPIC3.5 K − Δ3(E)-sd − Δ10(E)-sd − gcs) and negative control (pPIC3.5 K). Shown are representative chromatograms. a Detection of ion m/z = 774.5 and 776.5 representative of fusaruside and cerebroside B in FUS2, FUS3 and negative control strains, and in a 5 μg/mL standard solution, respectively. b MS spectra of fusaruside and cerebroside B, corresponding to the Na + −liganded molecular ions at m/z 774.5491 (774.5491 calcd. For C43H77NO9Na) and 776.5649 (776.5649 calcd. For C43H79NO9Na)