Fig. 4

Prediction of amino acid biosynthesis according to the metabolic annotation of P. irregulare CBS 494.86. The amino acid pathways are listed from A to F. The reactions are enumerated inside each specific pathway, followed by the encoded EC number. The EC numbers with a question mark (?) are cases of reactions not predicted by the metabolic annotation. Some metabolites and reactions are marked with M (metabolites) and/or numbers decoded as follows: in D- L-Alanine, L-Valine, Leucine & Iso-Leucine pathway, M.1 = (R)-3-Hydro 3-methyl 2-oxopentanoate, M.2 = (S)-2,3 Dihydroxy 3-methypentanoate, M.3 = (S)-3-Methyl 2-oxopentanoate, M.4 = 2-Isopropylmaleate, M.5 = (2R,3S)-3-Isopropylmalate, M.6 = (2S)-2-Isopropyl 3-oxopentanoate, M.7 = 4-Methyl 2-oxopentanoate; 3 = EC:1.1.1.86; 4 = EC: 1.1.1.86; 5 = EC: 4.2.1.9; 13 = EC: 2.3.3.13, 14 = EC: 4.2.1.33; 15 = EC: 1.1.1.85; 16 = Spontaneous; in E – L-Glutamate, L-Glutamine, Proline & Arginine pathway, M.8 = N-Acetyl-glutamate, M.9 = N-Acetyl-glutamyl-P, M.10 = N-Acetylglutamate semialdehyde, M.11 = N-Acetyl-ornithine, 5 = EC: 2.3.1.1; 6 = EC: 2.7.2.8; 7 = EC: 1.2.1.38; 8 = EC: 2.6.1.11; 9 = EC: 3.5.1.14; in E – L-Glutamine, L-Glutamate, Proline & Arginine pathway, M.12 = L-Glutamate 5-semialdehyde; M13 = (S)-1-Pyrroline-5-carboxylate;11 = non-enzymatic; 12 = EC:1.5.1.2; in F – L-Aspartate, L-Asparagine, Lysine, Threonine & L-Methionine pathway, M.14 = (2S,4S)-4-hydroxy 2,3,4,5 tetrahydro-dipicolinate, M.15 = L-2,3,4,5- Tetrahydro-dipicolinate, M.16 = L-L-2,6 Diamino-pimelate, M.17 = meso2,6 Diamino-pimelate, 13 = EC: 4.3.3.7;14 = EC: 1.17.1.8; 15 = EC: 2.6.1.83 (?); 16 = EC: 5.1.1.7 (?); 17 = EC: 4.1.1.20