Skip to main content

Table 1 Comparison of XenoLuc assay with commercial luminescent assays

From: Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system

Assay

Lytic/ Non-lytic

Price per reactiona

Incubation time

Signal intensity

Signal stability

Linearity (R2)

XenoLuc (In-house)

Lytic

RM 0.013 (USD 0.003)

15 min

36,133

>  1 h

0.9915

CellTiter-Glo (Promega, USA)

Lytic

RM 0.20 (USD 0.05)

1 h 15 min

165,000

>  1 h

0.9963

XenoLuc (In-house)

Non-lytic

RM 0.013 (USD 0.003)

10 min

120,960

>  1 h

0.999

RealTime-Glo (Promega, USA)

Non-lytic

RM 1.86 (USD 0.46)

1 h 15 min

279,400

>  1 h

0.9835

  1. aPrice acquired from Malaysia’s distributor