Fig. 2

Characterization of XenoLuc assay. a Diagram showing the basic concept of luciferase/luciferin system. b Comparison of signal intensity detected by XenoLuc assay and two commercial assays: CellTiter-Glo (lytic) and RealTime-Glo (non-lytic). c Comparison of signal stability of XenoLuc and RealTime-Glo. d Cytotoxicity of D-Luciferin substrate after 24-h incubation as determined by MTS assay. Results are represented by average of triplicate from 3 mice ± SD