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Fig. 1 | BMC Biotechnology

Fig. 1

From: Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system

Fig. 1

XenoLuc assay for in vitro cell proliferation measurement. a Measurement of luminescence in two-fold serially diluted non-depleted xenograft cells (parental and modified) and NHDF cells. These cells were seeded onto 96-well plates as 2D monolayer culture, and the luminescence was measured after 4 days. b Measurement of luminescence of mouse cell-depleted xenograft cells cultured in vitro in 2D and 3D models. c Proliferation rate of depleted xenografts cells as measured by XenoLuc assay at a 2-day interval. Results are represented by average of triplicate from 3 mice ± SD. Left panel: XenoB110-gfp-luc2; Right panel: Xeno284-gfp-luc2. R2 shows the linear correlation of luminescence and cell number

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BMC Biotechnology

ISSN: 1472-6750

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