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Table 2 Glucose, lactate, and glutamine content and pH of the ACF media 20 h after viral infection

From: Investigation of cell culture conditions for optimal foot-and-mouth disease virus production

virus A IRN/8/2015 NC A IRN/8/2015 NC A IRN/8/2015 NC
cell density 1 × 106 cells/mL 2 × 106 cells/mL 3 × 106 cells/mL
media exchange 100% 30% 30% 100% 30% 30% 100% 30% 30%
pH 7.5 ± 0.1 7.3 ± 0.1 7.1 ± 0.2 7.3 ± 0.1 7.0 ± 0.1 7.0 ± 0.1 7.2 ± 0.1 7.0 ± 0.3 6.9 ± 0.1
glucose (g/L) 5.2 ± 1.2 3.4 ± 1.0 2.6 ± 0.3 3.7 ± 1.8 1.6 ± 0.4 1.4 ± 0.6 2.6 ± 1.0 1.1 ± 0.5 1.2 ± 0.6
lactate (mM) 8.2 ± 0.9 23.0 ± 6.1 26.9 ± 4.6 16.6 ± 3.1 28.5 ± 4.1 29.2 ± 3.5 18.5 ± 2.6 25.1 ± 5.3 33.6 ± 4.1
glutamine (mM) 2.5 ± 1.8 1.5 ± 1.0 0.4 ± 0.3 2.5 ± 0.9 0.6 ± 0.6 0.1 ± 0.1 1.6 ± 0.6 0.3 ± 0.3 0.7 ± 0.9
virus isolate O SAU/18/2015 NC O SAU/18/2015 NC O SAU/18/2015 NC
cell density 1 × 106 cells/mL 2 × 106 cells/mL 3 × 106 cells/mL
media exchange 100% 30% 30% 100% 30% 30% 100% 30% 30%
pH 7.4 ± 0.0 7.4 ± 0.0 7.1 ± 0.0 7.4 ± 0.0 7.2 ± 0.2 7.0 ± 0.2 7.2 ± 0.20 6.9 ± 0.2 6.9 ± 0.2
glucose (g/L) 7.0 ± 1.6 5.2 ± 1.0 3.5 ± 1.0 5.1 ± 0.7 3.1 ± 1.2 2.1 ± 0.8 4.1 ± 0.3 1.5 ± 0.9 1.6 ± 0.5
lactate (mM) 7.5 ± 4.1 11.5 ± 5.8 21.4 ± 10.7 14.9 ± 6.8 23.7 ± 11.1 27.9 ± 14.0 16.6 ± 8.4 29.2 ± 14.9 36.8 ± 19.1
glutamine (mM) 4.3 ± 1.5 3.1 ± 0.9 1.3 ± 0.3 4.0 ± 1.3 1.6 ± 0.7 0.5 ± 0.4 2.6 ± 0.5 0.7 ± 0.3 0.2 ± 0.1