Fig. 6From: Investigation of cell culture conditions for optimal foot-and-mouth disease virus productionViral yield of BHK-2P cells at increasing densities and different media substitutions. BHK-2P cells were maintained in ACF media with increasing cell densities and a 30% or 100% media exchange before infection. Then cells were infected with FMDV A IRN/8/2015 (a) or O SAU/18/2015 (b). Viral titers for both isolates were significantly reduced at a cell density of 3 × 106 cells/mL if only 30% of media was exchanged. Experiments were performed three times independently and the viral yield was titrated in duplicate. Significance code: *** p < 0.001; **** p < 0.0001Back to article page