Recovery of recombinant Mycobacterium tuberculosis antigens fused with cell wall-anchoring motif (LysM) from inclusion bodies using non-denaturing reagent (N-laurylsarcosine)

Table 3 Primers used in this study

Fragment	Primer sequence (5′ to 3′)^a	Expected product size (base pair)
ARL	Forward GGATCCGAATTCGCTGACCAGCGAGCTGCCGC	1244
ARL	Reverse GCGGCCGCTTATTTTATTCGTAGATACTGAC
lysM	Forward CTGCAGCCATGGCGGCTGGAAGACGAGATGAAA	608
lysM	Reverse GCGGCCGCTTATTTTATTCGTAGATACTGAC
AR	Forward GGATCCGAATTCGCTGACCAGCGAGCTGCCGC	613
	Reverse GCGGCCGCTGGCTTCCCTTCCGAAACCGC
	Reverse CTGCAGGCTGGCTTCCCTTCCGAAACCGC
pRSF:Duet-1	Forward GGATCTCGACGCTCTCCCT	200
pRSF:Duet-1	Reverse TTGTACACGGCCGCATAATC

^aRestriction enzyme (RE) sites are shown as bold and underlined is either BamHI (GGATCC), PstI (CTGCAG) or NotI (GCGGCCGC)

ISSN: 1472-6750