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Table 2 Bacterial strains and plasmids used in this study

From: Recovery of recombinant Mycobacterium tuberculosis antigens fused with cell wall-anchoring motif (LysM) from inclusion bodies using non-denaturing reagent (N-laurylsarcosine)

Strain or plasmid Relevant Characteristics Source or reference
Strains
Lactococcus lactis MG1363 L. lactis subsp. cremoris; Lac, plasmid-free derivative of NCDO712 [23]
Lactobacillus plantarum Pa21 Malaysia local plant, Pandanus amaryllifoliusisolate, plasmid-free strain [31]
E. coli Rosetta (DE3)pLysS Camr, Expression host for all E. coli vectors, E. coli BL21 derivative Novagen
Plasmids
 pRSFDuet-1 3.8 kb, Kanr, E. coli expression vector Invitrogen
 pJET1.2/blunt 2.9 kb, Ampr, E. coli cloning vector, Thermo Scientific
 pJET:AR 3.5 kb, Ampr, pJET1.2 derivative carrying AR gene IDT
 pRSF:ARL 4.98 kb, Kanr, pRSFDuet-1 derivative carrying ARL fusion gene This study
 pRSF:AR 4.4 kb, Kanr, pRSFDuet-1 derivative carrying AR fusion gene This study