Stages of solubilization/purification | Total protein (mg)/0.1 g cell wet mass | Target protein (mg)/0.1 g cell wet mass | Purity (%) |
---|---|---|---|
Cell lysate | 3.21 | 1.53 | 47 |
Insoluble fraction/IBs | 1.9 | 1.14 | 60 |
Soluble fraction after 5% NLS treatment | 1.12 | 0.69 | 61.8 |
Purified ARL after Ni2+-NTA affinity chromatography | 0.64 | 0.63 | 98 |
Extractability (%) from IBs | 33.68% | 55.42% | – |