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Table 1 ARL protein recoveries (per 0.1 g wet cell mass) from inclusion bodies (IBs) by 5% (w/v) N-lauroylsarcosine (NLS) and its purification by Ni2+-NTA affinity chromatography

From: Recovery of recombinant Mycobacterium tuberculosis antigens fused with cell wall-anchoring motif (LysM) from inclusion bodies using non-denaturing reagent (N-laurylsarcosine)

Stages of solubilization/purification

Total protein (mg)/0.1 g cell wet mass

Target protein (mg)/0.1 g cell wet mass

Purity (%)

Cell lysate

3.21

1.53

47

Insoluble fraction/IBs

1.9

1.14

60

Soluble fraction after 5% NLS treatment

1.12

0.69

61.8

Purified ARL after Ni2+-NTA affinity chromatography

0.64

0.63

98

Extractability (%) from IBs

33.68%

55.42%

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