Fig. 5From: Recovery of recombinant Mycobacterium tuberculosis antigens fused with cell wall-anchoring motif (LysM) from inclusion bodies using non-denaturing reagent (N-laurylsarcosine)Stability analysis of the ARL protein anchored onto cell surface of L. plantarum. The cells were incubated with ARL protein and were subjected to ELISA using mouse anti-His monoclonal IgGs (Novagen, USA) as primary antibody and goat anti-mouse IgG-HRP (Calbiochem, USA) as secondary antibody for the duration of 5 days (Day 0 to Day 4). Negative control was L. plantarum incubated with PBSBack to article page