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Fig. 2 | BMC Biotechnology

Fig. 2

From: Recovery of recombinant Mycobacterium tuberculosis antigens fused with cell wall-anchoring motif (LysM) from inclusion bodies using non-denaturing reagent (N-laurylsarcosine)

Fig. 2

Expression analysis of the ARL protein in E. coli. Positive transformant cells harbouring pRSF:ARL were induced, lysed and analyzed for protein expression by sodium dodecyl sulfate polyacrylamide (SDS-PAGE). A control batch of bacterial cells harbouring pRSF:AR was also induced and analyzed to compare AR (~ 24 kDa) solubility to that of ARL (~ 45 kDa). Lanes 1–3, 4–6 and 7–9 correspond to the protein profile of uninduced cells harbouring pRSF:ARL, induced cells harbouring pRSF:ARL, and induced cells harbouring pRSF:AR, respectively. Lanes labeled with M, TP (lanes 1, 4 and 7), PF (lanes 2, 5 and 8) and SF (lanes 3, 6 and 9) refer to protein ladder marker (Fermentas, Canada), total protein, precipitant fraction and supernatant fraction, respectively

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BMC Biotechnology

ISSN: 1472-6750

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