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Table 1 Oligonucleotides used in this study

From: Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina

Oligonucleotide

Sequence (5′-3′)

pHG1A-for

CGAACGCATCGATTAATTAAGGAGGATCGTATGACGACCGTTCCCG

pHG1A-rev

CGTGGTGGGCGCTAGCCTCCTCTAGTCATCCGTCG

pHG1-for

ACTAGAGGAGGCTAGCTTCAATGGAGGAGATGATCGAGG

pHG1-rev

GCAGGTCGACTCTAGATCTCGCTACGCCGCTACG

pHG4-for

CGAACGCATCGATTAATTAAGCGGCCGCCATATGGAATTCGGTACCGCATGCAGATCTAGGAACTTCGAAGTTCCCGC

pHG4-rev

TGATTACGCCAAGCTTTCGACTCTAGAGTAAGCGTCACGG

pJH1R4-for

CTAGCGATTGCCATGACGTCGGAGCTGCTTACCAATGTC

pJH1R4-rev

AAGAGGCCCGCACCGATTCCAAGAGGCCGGCAACTAC

TG1-attB-Am-for

TCGATCTCCAGTGCGGGCAAGACGTTCAACTGCACCGGCTGGAAGATCGGGACCACCGGACGAACGCA

TG1-attB-Sa-for

TCGATCAGCTCCGCGGGCAAGACCTTCTCCTTCACGGGGTGGAAGGTCGGCGGTGGAGCTCGGAGA

R4-attB-Am-for

GGTTGCCCATCACCATGCCGAAGCAGTGATAGAAGGGAACCGGGATGCAGGTGAGAAGGTGCTCGTGT

R4-attB-Sp-for

AGTTGCCCATGACCATGCCGAAGCAGTGGTAGAAGGGCACCGGCAGACACGGTGAGAAGGTGCTCGTGT

attB-rev

CTGCATCTCAACGCCTTCCGG

TG1-attP-for

AACCTTCACGCTCATGCC

TG1-attP-rev

GTCGAGATTCTCCGTCTCCTG

R4-attP-for

GATCGGTCTTGCCTTGCTC

R4-attP-rev

ACCCGCAGAGTGTACCCA

TG1-attL-Am-for

ACAACCCCACCGGCACCGTCTTCA

TG1-attL-Am-rev

AGTATAGGAACTTCGAAGCAGCTC

R4-attL-Am-for

CGGCCGGTGATGTTGACGT

R4-attL-Am-rev

TCGGCCGTCACGATGGTCA

  1. The attB sequences are shown underlined