Fig. 3
From: Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina
PCR confirmation of site-specific integration in the exconjugants. (a) Integration of pHG4 into the chromosome. (b) PCR (using primers TG1-attL-Am-for/rev) of the expected TG1 attL-containing fragment from A. marina DSM45569:pHG4. M: Fast DNA Ladder. Colonies 1 to 6 are independent exconjugants. (c) Integration of pJH1R4 into the chromosome. (d) PCR (using primers R4-attL-Am-for/rev) of the expected R4 attL-containing fragment from A. marina DSM45569:pJH1R4. M: Fast DNA Ladder. Colonies 1 to 6 are independent exconjugants