Fig. 2
From: Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina
In vitro recombination. (a) Recombination substrates and their expected products. (b) In vitro recombination between DNA fragments containing TG1 attBAm (1627 bp) and TG1 attP (2471 bp; left), and R4 attBAm (1854 bp) and R4 attP (990 bp; right). The expected products of the TG1 integrase-mediated reaction were a 4.1 kb DNA fragment containing the attRAm site, and a 53 bp fragment containing attLAm (not observed). For the R4 integrase recombination reaction, the expected products were a 2.8 kb fragment containing attRAm, and a 51 bp attLAm fragment (not observed). (c) In vitro recombination between DNA fragments containing TG1 attBSa (1035 bp) and TG1 attP (2471 bp; left), and R4 attBSp (1855 bp) and R4 attP (990 bp; right). The expected products were a 3.5 kb fragment containing attRSa for the TG1 reaction, and a 2.8 kb fragment containing attRSp for the R4 reaction. M: Fast DNA Ladder (NEB, USA)