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Fig. 3 | BMC Biotechnology

Fig. 3

From: A novel autolysis system for extracellular production and direct immobilization of a phospholipase D fused with cellulose binding domain

Fig. 3

SDS-PAGE at each of the two steps for the extracellular expression of PLD-CBD. a M: molecular size markers. 1, 2: intracellular and extracellular products of pET28-PLD-CBD; 3, 4: intracellular and extracellular products of PLD-ESN-1; 5, 6: intracellular and extracellular products of PLD-ESN-2; 7, 8: intracellular and extracellular products of PLD-ESN-3; 9, 10: intracellular and extracellular products of PLD-ESN-4; 11, 12: intracellular and extracellular products of PLD-ESN-5. b M: molecular size markers. 1, unadsorbed crude enzyme solution. 2, purified enzyme solution eluted by 200 mM imidazole, the arrow means PLD-CBD

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BMC Biotechnology

ISSN: 1472-6750

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