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Table 1 Bacterial strains and plasmids used in this study

From: Mucosal delivery of Lactococcus lactis carrying an anti-TNF scFv expression vector ameliorates experimental colitis in mice

Plasmids Characteristics Reference
pValac Eukaryotic expression vector (pCMV/Cmr/RepA/RepC) [16]
pValac::anti-TNFα pValac containing anti-TNFα scFv ORF This study.
Bacterial strain and plasmids Characteristics Reference
Escherichia coli XL1-Blue E. coli; F′[Tn10 proAB+ lacIq Δ (lacZ)M15] hsdR17(rK mK+)/ Tetr Stratagene® (Catalog no #200249)
Escherichia coli TG1 E. coli, K-12-derived strain; F′ [traD36 proAB+ lacIq lacZΔM15] supE thi-1 Δ (lac-proAB) Δ (mcrB-hsdSM)5, (rKmK) Lucigen, Middleton, MI, USA (Catalog no 60502–1)
Lactococcus lactis MG1363 FnBPA+ (LL-F) L. lactis MG1363 strain expressing FnBPA of S. aureus (Eryr) [15], obtained from Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais (UFMG), Brazil,
Lactococcus lactis MG1363 FnBPA+ (pValac::anti-TNFα) (LL-FT) L. lactis MG1363 FnBPA+ strain carrying the pValac::anti-TNFα plasmid (Cmr/ Eryr) This study.
  1. Cmr chloramphenicol resistance; Tetr tetracycline resistance; Eryr erythromicin resistance