Table 1 Bacterial strains and plasmids used in this study

Plasmids

Characteristics

Reference

pValac

Eukaryotic expression vector (pCMV/Cm^r/RepA/RepC)

[16]

pValac::anti-TNFα

pValac containing anti-TNFα scFv ORF

This study.

Bacterial strain and plasmids

Characteristics

Reference

Escherichia coli XL1-Blue

E. coli; F′[Tn10 proAB⁺ lacI^q Δ (lacZ)M15] hsdR17(r_K⁻ m_K⁺)/ Tet^r

Stratagene® (Catalog n^o #200249)

Escherichia coli TG1

E. coli, K-12-derived strain; F′ [traD36 proAB⁺ lacI^q lacZΔM15] supE thi-1 Δ (lac-proAB) Δ (mcrB-hsdSM)5, (r_K⁻m_K⁻)

Lucigen, Middleton, MI, USA (Catalog n^o 60502–1)

Lactococcus lactis MG1363 FnBPA+ (LL-F)

L. lactis MG1363 strain expressing FnBPA of S. aureus (Ery^r)

[15], obtained from Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais (UFMG), Brazil,

Lactococcus lactis MG1363 FnBPA+ (pValac::anti-TNFα) (LL-FT)

L. lactis MG1363 FnBPA+ strain carrying the pValac::anti-TNFα plasmid (Cm^r/ Ery^r)

This study.