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Table 3 Summary of genotypes in Ig Heavy chain gene, AR gene and RBFOX1 gene in embryos injected with 2.5 ng/μL sgRNA and 5 ng/μL Cas9 mRNA. Seven embryos were analyzed for off-targeting events. Heterozygous: WT and one modified allele; Biallelic: two differently modified alleles; Homozygous: one type of modified alleles; Mosaic: more than two types of alleles and the WT sequence may or may not be present

From: Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

Embryo #

Ig Heavy Chain

AR

RBFOX1

1

Biallelic

Heterozygous

WT

2

Heterozygous

Heterozygous

Heterozygous

3

Homozygous

Mosaic w/o WT

WT

4

Homozygous

WT

Mosaic w/ WT

5

Biallelic

Heterozygous

Heterozygous

6

Biallelic

Heterozygous

Heterozygous

7

Mosaic w/o WT

Heterozygous

Mosaic w/ WT