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Fig. 2 | BMC Biotechnology

Fig. 2

From: Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

Fig. 2

Chromatogram showing potential off-target activity on RBFOX1 and AR gene from Ig Heavy chain knockout pig. Similar approach was used to identify any off-target events on RAG2/IL2RG double knockout pigs or SCD5 knockout fetuses. a WT sequence of RBFOX1. b Polymorphic reading from Ig Heavy chain knockout pig E. c Allele specific reading from Ig Heavy chain knockout pig E. d WT sequence of AR. e Polymorphic reading from Ig Heavy chain knockout pig E. f Allele specific reading from Ig Heavy chain knockout pig E. Yellow highlighted portion of sequence indicates deviation from WT sequence reading

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BMC Biotechnology

ISSN: 1472-6750

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