Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9 based gene editing

BMC Biotechnology

Table 2 The number of mutations induced from transformed roots and transfected protoplasts by gene editing in the FAD2 genes

Genotype	Transformed root event	G448A	441_442insA	G451T	No mutation	Total number
Exp27–15,16	10	37^a	13	28	22	100
GT-C20	10	10				10
14 AU-01	Transfected protoplasts	16^a		18	12	46
Total reads		63	13	46	34	156

^amutation G448A was naturally existed in these two genotypes, not induced by gene editing

ISSN: 1472-6750