Fig. 3

Functional validation of TRE3G-cMyc transfer vector in c-Myc knock-out cells HO15.19. Cells modified and purified with puromycin were cultured for 4 days with various dox concentrations. Growth for all conditions was measured relative to the no vector control with no dox. Relative growth was measured as relative fluorescence using the viability assay reagent AlamarBlue at 4 days which assumes AlamarBlue conversion is linearly proportional to cell number