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Fig. 6 | BMC Biotechnology

Fig. 6

From: Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5

Fig. 6

Transduction of CD4+ or CD8+ T cells by fiber-modified HAdV-5 vectors. T cells were isolated, expanded and infected by adenoviral vectors at MOIs of 500 or 1000 vp/cell. Two days post infection, cells were labelled with fluorescein-conjugated anti-CD3, anti-CD4 and anti-CD8 antibodies. GFP and fluoresceins were analyzed with flow cytometry. The data processing procedure was shown (a). Live lyphocytes were gated and separated from the cellular debris on the FSC-H vs. SSC-H dot plot, and CD3+ T cells were then gated and grouped according to the expression of CD4 or CD8 molecules. GFP expression in CD4 + CD8- or CD4-CD8+ subgroups were separately analyzed (b), and the percentages of CD4 + CD8- or CD4-CD8+ cells in CD3+ T cells were calculated (c)

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