Fig. 1
From: Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5
Schematic diagram of the construction of fiber-modified human adenovirus 5 (HAdV-5) vectors. a Construction of the shuttle plasmid carrying human EF1a promoter. b Construction of the backbone plasmid carrying modified fiber gene. c Fiber-modified HAdV-5 vectors.All vectors contained the same human EF1a promoter controlled GFP expression cassette inserted into the E1 region and differently modified fiber genes. CMVp, CMV promoter; C-RGD, RGD4C fused to the C-terminus of HAdV-11p fiber; EF1ap, human EF1a promoter; ES, encapsidation signal; HI-RGD, RGD4C inserted into the HI loop of HAdV-11p fiber Knob; ITR, inverted terminal repeat; Knob11p, knob of HAdV-11p fiber; Knob35, knob of HAdV-35 fiber; MCS, multiple cloning site; pA, SV40 polyA signal; Shaft11p, shaft of HAdV-11p fiber; Shaft35, shaft of HAdV-35 fiber; Tail5, tail of HAdV-5 fiber