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Table 3 Enzyme kinetic comparison of AFases characterised in this study and thermostable AFases obtained from literature

From: Characterisation of three novel α-L-arabinofuranosidases from a compost metagenome

AFase

Micro-organism

KM in mM (Std. error)

Sp. act. in U/mg (Std. error)

kcat (s−1)

kcat/KM (M−1 s−1)

Reference

AFase-H4

Unknown

0.31 (±0.03)

143.1 (±3.7)

131

4.2 × 105

This study

AFase-E3

Unknown

0.33 (±0.04)

228.6 (±9.7)

219

6.6 × 105

This study

AFase-D3

Unknown

0.38 (±0.05)

166.6 (±6.9)

155

4.1 × 105

This study

abf51S9

Streptomyces sp. S9

1.45

221

203

1.4 × 105

[29]

AbfATK4

Geobacillus caldoxylolyticus TK4

0.17

588

568

3.3 × 106

[30]

AbfAC26Sari

Anoxybacillus kestanbolensis

0.14

1019

968

7 × 106

[31]

Tm-Afase

Thermotoga maritima

0.42

23.5

22

5.2 × 104

[42]

AFase

Caldocellulosiruptor sacchrolyticus

1.29

295

285

2.2 × 105

[60]

abfB

Auriobasidium pullulans

6.27

78.1

96.3

1.5 × 104

[62]