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Table 3 Enzyme kinetic comparison of AFases characterised in this study and thermostable AFases obtained from literature

From: Characterisation of three novel α-L-arabinofuranosidases from a compost metagenome

AFase Micro-organism KM in mM (Std. error) Sp. act. in U/mg (Std. error) kcat (s−1) kcat/KM (M−1 s−1) Reference
AFase-H4 Unknown 0.31 (±0.03) 143.1 (±3.7) 131 4.2 × 105 This study
AFase-E3 Unknown 0.33 (±0.04) 228.6 (±9.7) 219 6.6 × 105 This study
AFase-D3 Unknown 0.38 (±0.05) 166.6 (±6.9) 155 4.1 × 105 This study
abf51S9 Streptomyces sp. S9 1.45 221 203 1.4 × 105 [29]
AbfATK4 Geobacillus caldoxylolyticus TK4 0.17 588 568 3.3 × 106 [30]
AbfAC26Sari Anoxybacillus kestanbolensis 0.14 1019 968 7 × 106 [31]
Tm-Afase Thermotoga maritima 0.42 23.5 22 5.2 × 104 [42]
AFase Caldocellulosiruptor sacchrolyticus 1.29 295 285 2.2 × 105 [60]
abfB Auriobasidium pullulans 6.27 78.1 96.3 1.5 × 104 [62]