Table 1 Bacterial strains, plasmid vectors and their recombinant versions used in this study
From: Characterisation of three novel α-L-arabinofuranosidases from a compost metagenome
Strain | Genotypea | Reference |
|---|---|---|
E. coli Epi300 | F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74recA1 endA1 araD139 (ara, leu) 7697 galK1-rspLnupGtrfr | Invitrogen, USA |
Genehog | F-mcrA ∆(mrr-hsdRMS-mcrBC) φ80dlacZ∆M15 ∆lacX74recA1 araD139 ∆(ara, leu) 7697 galUgalKrpsL (StrR(endA1 nupGfhuAIS2r | Epicentre Biotechnology, USA |
BL21 (DE3) | F-ompThsdSB(rB-mB-)gal dcm gal ʎ(DE3) | Invitrogen, USA |
pCC1Fos™ | pCC1Fos™L-Arabinose inducible promoter Copy Control; CamR, F factor ori, oriV high copy ori, λcos site for λ packaging, Bacteriophage T7 RNA polymerase promoter | Invitrogen, USA |
pFos-H4 | pCC1FOS containing 17.5 kb of cloned metagenomic DNA as an insert with AFase activity, CamR | IMBM |
pFos-E3 | pCC1FOS containing 20.7 kb of cloned metagenomic DNA as an insert with AFase activity, CamR | Dr C. Ohlhoff, IMBM, UWC, SA |
pFos-D3 | pCC1FOS containing 10. 7 kb of cloned metagenomic DNA as an insert with AFase activity, CamR | Dr C. Ohlhoff, IMBM, UWC, SA |
pJET 1.2/blunt | Suicide cloning vector (eco47IR), blunt DNA ends for ligation with insert, T7 promoter, AmpR | Fermentas, USA |
pJET-H4 | 1467 bp AFase-H4 gene amplicon blunt-end ligated into pJet1.2 | This study |
pJET-E3 | 1547 bp AFase-E3 gene amplicon blunt-end ligated into pJet1.2 | This study |
pJET-D3 | 1482 bp AFase-D3 gene amplicon blunt-end ligated into pJet1.2 | This study |
pET21a(+) | Expression vector with a C- terminal His-tag, AmpR, T7 promoter and terminator, MCS. | Novagen, USA |
pET21a-H4 | 1467 bp NdeI-XhoI fragment from pJET-H4 cloned in pET21a. | This study |
pET21a-E3 | 1547 bp NdeI- HindIII fragment from pJET-H4 cloned in pET21a. | This study |
pET21a-D3 | 1482 bp NdeI-XhoI fragment from pJET-H4 cloned in pET21a. | This study |