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Table 1 Bacterial strains, plasmid vectors and their recombinant versions used in this study

From: Characterisation of three novel α-L-arabinofuranosidases from a compost metagenome

Strain Genotypea Reference
E. coli Epi300 F-mcrA D(mrr-hsdRMS-mcrBC) f80dlacZDM15 DlacX74recA1 endA1 araD139 (ara, leu) 7697 galK1-rspLnupGtrfr Invitrogen, USA
Genehog F-mcrA ∆(mrr-hsdRMS-mcrBC) φ80dlacZ∆M15 ∆lacX74recA1 araD139 ∆(ara, leu) 7697 galUgalKrpsL (StrR(endA1 nupGfhuAIS2r Epicentre Biotechnology, USA
BL21 (DE3) F-ompThsdSB(rB-mB-)gal dcm gal ʎ(DE3) Invitrogen, USA
pCC1Fos™ pCC1Fos™L-Arabinose inducible promoter Copy Control; CamR, F factor ori, oriV high copy ori, λcos site for λ packaging, Bacteriophage T7 RNA polymerase promoter Invitrogen, USA
pFos-H4 pCC1FOS containing 17.5 kb of cloned metagenomic DNA as an insert with AFase activity, CamR IMBM
pFos-E3 pCC1FOS containing 20.7 kb of cloned metagenomic DNA as an insert with AFase activity, CamR Dr C. Ohlhoff, IMBM, UWC, SA
pFos-D3 pCC1FOS containing 10. 7 kb of cloned metagenomic DNA as an insert with AFase activity, CamR Dr C. Ohlhoff, IMBM, UWC, SA
pJET 1.2/blunt Suicide cloning vector (eco47IR), blunt DNA ends for ligation with insert, T7 promoter, AmpR Fermentas, USA
pJET-H4 1467 bp AFase-H4 gene amplicon blunt-end ligated into pJet1.2 This study
pJET-E3 1547 bp AFase-E3 gene amplicon blunt-end ligated into pJet1.2 This study
pJET-D3 1482 bp AFase-D3 gene amplicon blunt-end ligated into pJet1.2 This study
pET21a(+) Expression vector with a C- terminal His-tag, AmpR, T7 promoter and terminator, MCS. Novagen, USA
pET21a-H4 1467 bp NdeI-XhoI fragment from pJET-H4 cloned in pET21a. This study
pET21a-E3 1547 bp NdeI- HindIII fragment from pJET-H4 cloned in pET21a. This study
pET21a-D3 1482 bp NdeI-XhoI fragment from pJET-H4 cloned in pET21a. This study
  1. a Plasmid encoding Ampicillin and Chloramphenicol resistance is indicated as AmpR and CamR, respectively. Ori is used as an abbreviation for origin of replication and MCS is the acronym for multiple cloning site. The Hexa-histidine tag is abbreviated to His-tag that was used to purify the AFase proteins of this study through nickel-ion affinity liquid chromatography