Fig. 2

Proportion of native gene copy-deleted clones obtained after the CRISPR-Cas9 approach targeting ‘bait’ DNA. A CRISPR-Cas9 approach targeting ‘bait’ DNA was performed to delete lsr2A or lsr2B paralogs in S. ambofaciens WT or related strains containing an extracopy of lsr2 paralog (WT attPhiC31ΩpSET152-lsr2X, X being either A or B). The WT strain containing an empty vector (WT attPhiC31ΩpSET152) was used as a control. The number of hygromycin-sensitive clones (i.e. having lost the hygromycin resistance gene after CRISPR-Cas9 targeting) harboring a native or a deleted lsr2A or lsr2B gene was determined by PCR analysis. The p₁ value represents the p value obtained from a Fisher’s Exact test for count data comparing the frequencies of deletion to the WT condition (for a given lsr2 paralog CRISPR-Cas9 approach). The p₂ value represents the p value from the same test performed to compare the frequencies of deletion to a theoretical frequency of 0.5 (using the same total effective as reference). Abbreviation: ns = not statistically significant