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Fig. 2 | BMC Biotechnology

Fig. 2

From: Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase

Fig. 2

α-AIC3 purification through size exclusion chromatography. a- Chromatogram generated from molecular size exclusion chromatography of α-AIC3-expressing N. benthamiana extracts after dialysis against water. The indicated peaks comprise fractions 10–14, 16–20 and 30–58. A total of 180 mL of eluted volume was obtained, distributed in 90 fractions of 2 mL each. Software: UNICORN™ 6.4 (GE Healthcare). b- Silver-stained 15% SDS-PAGE of selected SEC fractions (15 μL). CE: crude extract; W: washing; numbers: selected SEC fractions. c- Western blot of 15% SDS-PAGE gel using a specific primary anti-α-AIC3 antibody. Sample analysed consists of combined fractions 17, 18 and 19 of purified and concentrated α-AIC3. The four bands analysed by mass spectrometry are indicated. M: Molecular marker

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BMC Biotechnology

ISSN: 1472-6750

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