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Fig. 1 | BMC Biotechnology

Fig. 1

From: Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase

Fig. 1

Detection of α-AIC3 expression in presence of gene silencing suppressor combination (P0, P1 and P19). a- Coomassie Blue-stained 15% SDS-PAGE consisting of 40 μg of total protein from crude extracts of pBIN61 samples (1) and pBIN61:α-AIC3 samples (2) from N. benthamiana leaves co-expressing these vectors with the three gene silencing suppressors . b- Western blot of corresponding Coomassie Blue-stained gel using a specific primary anti-α-AIC3 antibody. Expected bands for whole and unprocessed α-AIC3 (27 kDa), as well as for its subunits (α-subunit, 12 kDa, and β-subunit, 15 kDa), are shown. M: Molecular marker

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BMC Biotechnology

ISSN: 1472-6750

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