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Table 2: CRISPR-X-mediated evolution of A2Ab: NGS analysis, round 2

From: Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

R2
Cluster name mAb name %R2+ (counts) %R2- (counts)
D74H//S80 T//W102 L//M112I//G121D//R124P C4.4 49.2 (14755) 9.2 (2756)
D74H//S80 T//W102 L//D109A//M112I//G121D//R124P   2.4 (733) 0.2 (73)
D74H//S80 T//M112I//G121D//R124P   2.2 (650) 0
G121E   1.7 (496) 38.9 (11670)
D74H//S80 T//F83S//W102 L//M112I//G121D//R124P   0.7 (223) 0.4 (112)
D74H//S80 T//A98P//W102 L//M112I//G121D//R124P   0.6 (182) 0
D74H//S80 T//W102 L//M112I//G121D//R124P//V140 L   0.6 (173) 0
D74H//W102 L//M112I//G121D//R124P C4.18 0.5 (163) 0
G121D//R124P   0.2 (74) 0.9 (274)
D74H//S80 T//W102 L//S104 T//M112I//G121D//R124P   0.2 (72) 0
D74H//S80 T//W102 L//G121E   0.2 (68) 0
W102 L//M112I//G121D//R124P   0.2 (63) 4.2 (1247)
D74H//S80 T//W102 L//L105R//M112I//G121D//R124P   0.2 (59) 0
W52C//G121E   0 1.1 (333)
G121E//V140 L   0 0.7 (209)
R47S//R57H//G121E   0 0.7 (204)
W102 L//G121E   0 0.7 (203)
WT   0 0.6 (193)
M112I//G121D//R124P A2Ab 0 0.5 (137)
W102 L//M112I//G121E   0 0.4 (128)
H101Q//G121E   0 0.4 (122)
I39M//H101Q//G121E   0 0.4 (119)
P60S//G121E   0 0,4 (81)
C41Y//G121E   0 0.2 (66)
I39M//G121E   0 0.2 (56)
W102C//G121E   0 0.2 (56)
small clusters R2+ (number)   41 (12289)  
small clusters R2- (number)    39.9 (11961)
total   100 (30000) 100 (30000)