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Table 1 CRISPR-X-mediated evolution of A2Ab: NGS analysis, round 1

From: Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

R1

Cluster name

mAb name

%R1+ (counts)

%R1- (counts)

G121E

C3.2

31.8 (9542)

0.3 (94)

WT

A2Ab

13.6 (4103)

52.6 (15788)

W102 L//M112I//G121D//R124P

C3.9

10.7 (3197)

0

G121E//V140 L

 

1.1 (340)

0

G121D

C3.3

1.1 (316)

0.3 (95)

S103 N//G121D

C3.5

0.9 (261)

0

W102 L//D109A//M112I//G121D//R124P

 

0.8 (239)

0

M112I//G121D//R124P

 

0.7 (209)

0

V140 L

 

0,6 (168)

1.5 (448)

R117S

 

0

0.5 (148)

Y114S

 

0

0.4 (124)

D109A

 

0

0.4 (119)

S103R

 

0

0.2 (67)

S108A

 

0

0.2 (66)

G137R

 

0

0.2 (61)

R119S

 

0

0.2 (60)

P60A

 

0

0.2 (54)

V123G

 

0

0.2 (54)

small clusters R1+ (number)

C3.4

38.7 (11625)

 

small clusters R1- (number)

  

42.7 (12817)

total

 

100 (30000)

100 (30000)