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Table 1 CRISPR-X-mediated evolution of A2Ab: NGS analysis, round 1

From: Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

R1
Cluster name mAb name %R1+ (counts) %R1- (counts)
G121E C3.2 31.8 (9542) 0.3 (94)
WT A2Ab 13.6 (4103) 52.6 (15788)
W102 L//M112I//G121D//R124P C3.9 10.7 (3197) 0
G121E//V140 L   1.1 (340) 0
G121D C3.3 1.1 (316) 0.3 (95)
S103 N//G121D C3.5 0.9 (261) 0
W102 L//D109A//M112I//G121D//R124P   0.8 (239) 0
M112I//G121D//R124P   0.7 (209) 0
V140 L   0,6 (168) 1.5 (448)
R117S   0 0.5 (148)
Y114S   0 0.4 (124)
D109A   0 0.4 (119)
S103R   0 0.2 (67)
S108A   0 0.2 (66)
G137R   0 0.2 (61)
R119S   0 0.2 (60)
P60A   0 0.2 (54)
V123G   0 0.2 (54)
small clusters R1+ (number) C3.4 38.7 (11625)  
small clusters R1- (number)    42.7 (12817)
total   100 (30000) 100 (30000)