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Fig. 2 | BMC Biotechnology

Fig. 2

From: Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

Fig. 2

Schematic illustration of CRISPR-X. a dCas9 associated with a sgRNA containing MS2 hairpins recruits AID*Δ fused to MS2 coat protein leading to localized mutations (stars). Mutations can be induced in the sgRNA binding site or upstream or downstream from it, though only downstream mutations are illustrated here. b Binding sites for the nine sgRNAs used on the A2Ab heavy chain variable domain coding sequence are shown. Blue and orange colors indicate complementarity to non-coding and coding strands respectively

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