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Table 2 Analysis of targeted gene mutations induced by CRISPR-Cas9 cassettes in transgenic soybean lines. Transgenic plants containing confirmed FAD2–2 mutagenesis were subjected to look for off target editing

From: CRISPR-Cas9 mediated targeted disruption of FAD2–2 microsomal omega-6 desaturase in soybean (Glycine max.L)

SgRNA No. of putative off target sites No. of loci of putative off target sites No. of examined loci No. of plants examined No. of off target mutation
FAD2–2-sgRNA2 20 19 12 8 0