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Table 1 Mutation efficiency of FAD2–2 gene induced by CRISPR-Cas9 system

From: CRISPR-Cas9 mediated targeted disruption of FAD2–2 microsomal omega-6 desaturase in soybean (Glycine max.L)

Target gene No. of examined plant No. of plants with cas9 gene No. of plants with mutations Mutation efficiency %
FAD2–2 556 37 8 21%
  1. Mutation efficiency (%) = No of plants with mutations/No of plants with Cas9 gene×100