Characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803

Table 4 IC₅₀ of each parent and plasmid addition strain and percent differences of tolerance relative to the parent strain

Treatment		SD100	SD100 pacrA	SD100 pKan	SD100 pacrB	SD100 pSpec
Amp	IC₅₀	955.2 ± 256	1315 ± 77 (ns)	1151 ± 82 (ns)	1109 ± 123 (ns)	658.6 ± 88.3
Amp	% diff from SD100	0%	27%	20%	16%	−31%
Cm	IC₅₀	2.290 ± 0.401	1.444 ± 0.114	1.390 ± 0.186	1.976 ± 0.734 (ns)	1.653 ± 0.205 (ns)
Cm	% diff from SD100	0%	−59%	−39%	−14%	−28%
Ery	IC₅₀	0.358 ± 0.081 μg/mL	0.080 ± 0.003	0.072 ± 0.007	0.138 ± 0.080 (ns)	0.079 ± 0.005
Ery	% diff from SD100	0%	−77%	− 79%	−61%	−78%
Treatment		SD277	SD277 pacrA	SD277 pKan	SD277 pacrB	SD277 pSpec
Amp	IC₅₀	1570 ± 170 μg/mL	1117 ± 21 μg/mL	76.67 ± 28.5 μg/mL	118.2 ± 8.7 μg/mL	412.6 ± 34.8 μg/mL
Amp	% diff from SD277	0%	−29%	−95%	−92%	−74%
Cm	IC₅₀	4.749 ± 1.043 μg/mL	1.833 ± 0.390 μg/mL	1.793 ± 0.430 μg/mL	1.984 ± 0.527 μg/mL	2.96 ± 0.4 μg/mL
Cm	% diff from SD277	0%	−61%	− 62%	−58%	−38%
Ery	IC₅₀	0.427 ± 0.249 μg/mL	0.091 ± 0.034 μg/mL	0.149 ± 0.016 μg/mL	0.080 ± 0.008 μg/mL	0.055 ± 0.004 μg/mL
Ery	% diff from SD277	0%	−79%	−65%	−81%	−87%

The basis for the comparison in this table were the parent strains, either SD100 or SD277, featured in the third column. Each derivative strain is significantly different from the parent strain unless otherwise noted with “(ns)”

ISSN: 1472-6750