Fig. 2From: Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complexIn vitro digestion of cbh1 by Cas9 and gRNAs. M: DNA molecular mass marker; lane 1: BcuI-linearized plasmid; lane 2–4: BcuI-linearized plasmid incubated with Cas9 and gRNA1 (lane 2), gRNA2 (lane 3), and gRNA3 (lane 4)Back to article page