Table 1 Transformation rates of ten microalgal strains by Agrobacterium-mediated transformation
Species | Algal media | Concentration of hygromycin B (μg ml− 1) | Selection period (days) | Transformation rate (CFU per 106 cells) | Background control (CFU per 106 cells) |
|---|---|---|---|---|---|
Coelastrum sp. (TISTR 8511) | TAP | 10 | 15 | 0 (n = 44) | 0 |
S. acutus (TISTR 8555) | TAP | 30 | 15 | 20 ± 5.5 (n = 12) | 1.5 ± 1.3 |
S. acutus (TISTR 8540) | TAP | 50 | 10 | 72 ± 41 (n = 8) | 3.7 ± 3.5 |
S. acuminatus (TISTR 8519) | TAP | 50 | 10 | 31 ± 23 (n = 11) | 2.7 ± 2.5 |
S. acutus (TISTR 8447) | TAP | 30 | 15 | 217.5 ± 75 (n = 12) | 19.5 ± 6.8 |
C. humicola (TISTR 8434) | TAP | 20 | 15 | 10.8 ± 32 (n = 4) | 0 |
M. braunii (TISTR 8429) | BG-11 | 10 | 15 | 0 (n = 8) | 0 |
A. falcatus (TISTR 8557) | BG-11 | 10 | 15 | 0 (n = 12) | 0 |
T. cumbricus (TISTR 8480) | TAP | 50 | 18 | 9 ± 8.8 (n = 5) | 0 |
A. densus (TISTR 8505) | BG-11 | 10 | 15 | 0 (n = 8) | 0 |