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Table 1 Transformation rates of ten microalgal strains by Agrobacterium-mediated transformation

From: Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus

Species Algal media Concentration of hygromycin B (μg ml− 1) Selection period (days) Transformation rate (CFU per 106 cells) Background control (CFU per 106 cells)
Coelastrum sp. (TISTR 8511) TAP 10 15 0 (n = 44) 0
S. acutus (TISTR 8555) TAP 30 15 20 ± 5.5 (n = 12) 1.5 ± 1.3
S. acutus (TISTR 8540) TAP 50 10 72 ± 41 (n = 8) 3.7 ± 3.5
S. acuminatus (TISTR 8519) TAP 50 10 31 ± 23 (n = 11) 2.7 ± 2.5
S. acutus (TISTR 8447) TAP 30 15 217.5 ± 75 (n = 12) 19.5 ± 6.8
C. humicola (TISTR 8434) TAP 20 15 10.8 ± 32 (n = 4) 0
M. braunii (TISTR 8429) BG-11 10 15 0 (n = 8) 0
A. falcatus (TISTR 8557) BG-11 10 15 0 (n = 12) 0
T. cumbricus (TISTR 8480) TAP 50 18 9 ± 8.8 (n = 5) 0
A. densus (TISTR 8505) BG-11 10 15 0 (n = 8) 0