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Fig. 4 | BMC Biotechnology

Fig. 4

From: Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus

Fig. 4

Generation of psy white mutants and complementation using CrPSY. a Six isolated white mutants (G1-6) on TAP medium under light and dark conditions (No.6: TISTR8447, 4A+: Chlamydomonas, cc4113 and cc4109: Chlamydomonas psy white mutants). b Genotyping of psy mutants by direct PCR and sequencing. c Complementation of psy white mutants using CrPSY coding sequence fused in-frame with E2A and mCherry. mCherry was detected by both fluorescence microplate reader and immunoblot analysis (m: mCherry from E. coli, wt: TISTR8447). mCherry carrying HA tag in the algae is 28.4 kDa, while that in E. coli is 26.7 kDa. A fainted band representing 74 kDa of CrPSY::E2A::mCherry fusion protein was observed (see Additional file 8 for longer exposures and Additional file 10 for the full length blot)

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