Fig. 2

Analysis of TISTR8447 transformants expressing transcript fusions. a pCreZ-ble::E2A::mCherry (ble) and pCreZ-intble::E2A::mCherry (int-ble) vectors are illustrated. b TISTR8447 transformants analyzed by confocal microscopy showing mCherry signal and FITC channel-autofluorescence (scale bar = 20 μm). E. coli expressing mCherry was added to TISTR8447 wild type as a positive control. c mCherry signals of ble and int-ble vector transformants are presented as box plots overlaid with scatter plots. (n) indicates the number of transformants. d Fluorescence signals of low, medium and high mCherry expressors from each construct after subjected to media supplemented with 0–50 μg ml^− 1 zeocin for 2 days. Error bars represent standard deviations (n = 3)