Table 3 The positive rates of EmGFP knock-in demonstrated by PCR analysis or GFP detection
From: Homologous recombination-mediated targeted integration in monkey embryos using TALE nucleases
Method | Number of zygote injected with plasmid | Number of positive embryos | positive rate |
|---|---|---|---|
PCR analysis | 62 | 7 | 11.3% |
GFP detection | 9 | 1 | 11.1% |