Table 2 Primers used for genotyping
From: Homologous recombination-mediated targeted integration in monkey embryos using TALE nucleases
Primer | Sequence (5′ to 3′) | Usage | Amplicon(bp) |
|---|---|---|---|
L1-F | AGGATCGCTTGAGGACAGGAATT | PCR | 3259 bp (5’gDNA~ 3’gDNA) |
L1-R | GGAGCCATCACAGGAGACAGAAAA | PCR/sequencing | |
S1-F | GATGCATTGAGGGATAGCTCCACACACACATTC | PCR/sequencing | 1795 bp (5’ gDNA ~EmGFP) |
S1-R | AGGACCATGTGATCGCGCTTCT | PCR | |
S2-F | CAGAAGAACGGCATCAAG | PCR | 2109 bp (EmGFP~ 3’gDNA) |
S2-R | AGTAGGGAGGAAGCAGTAGAA | PCR | |
G-F | CACAAGTTCAGCGTGTCCG | PCR | 421 bp (EmGFP) |
G-R | AGTTCACCTTGATGCCGTTC | PCR | Â |
LI-W1R-G08 | ATCCCTCAGCAGCTCTACATTT | sequencing | Â |
L1-W2R-H11 | CAGCTTCACGGCACCAGTAC | sequencing | Â |
L1-W3R-C04 | GTAGCGGGCGAAGCACTG | sequencing | Â |
S12-W1R-C01 | TCCGAGGATCAACCCAGC | sequencing | Â |
OT-F | CACCACCATTAGGCAAAC | PCR | 1201 bp target site included |
OT-R | GAGGCTGAAGTCAATCAAAA | PCR | Â |