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Table 1 Strains used in this study

From: Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Strain Characteristics Source
E. coli EPI300 Gene cloning host Epicentre Technologies Corp, USA
P. pastoris KM71 Gene expression host with histidine deficiency Invitrogen, USA
P. pastoris KM71H Gene expression host Invitrogen, USA
P. pastoris KM71/GFP GFP expressed in P. pastoris KM71 and histidine deficiency was restored by the empty pAOX2 vector In this study
P. pastoris KM71m/GFP GFP expressed in P. pastoris KM71m containing the synthetic Mxr1 circuit In this study
P. pastoris KM71H/scFv scFv expressed in P. pastoris KM71H In this study
P. pastoris KM71Hm/scFv scFv expressed in P. pastoris KM71Hm containing the synthetic Mxr1 circuit In this study