Skip to main content

Table 1 Strains used in this study

From: Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Strain

Characteristics

Source

E. coli EPI300

Gene cloning host

Epicentre Technologies Corp, USA

P. pastoris KM71

Gene expression host with histidine deficiency

Invitrogen, USA

P. pastoris KM71H

Gene expression host

Invitrogen, USA

P. pastoris KM71/GFP

GFP expressed in P. pastoris KM71 and histidine deficiency was restored by the empty pAOX2 vector

In this study

P. pastoris KM71m/GFP

GFP expressed in P. pastoris KM71m containing the synthetic Mxr1 circuit

In this study

P. pastoris KM71H/scFv

scFv expressed in P. pastoris KM71H

In this study

P. pastoris KM71Hm/scFv

scFv expressed in P. pastoris KM71Hm containing the synthetic Mxr1 circuit

In this study