Fig. 5From: Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1Synthetic circuit of Mxr1 enhanced scFv expression. After being cultured in BMGY (1% glycerol), cells were cultured in BMMY and induced with 1% methanol daily. a The relative secreted scFv titre is presented. The error bars represent the standard deviation of three biological replicates. One-way ANOVA and the Tukey test were used to determine significance. *, p < 0.05; ***, p < 0.005. b Intracellular proteins after induction were examined by western blotting using an anti-his antibody. GAPDH was used as the loading controlBack to article page