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Fig. 5 | BMC Biotechnology

Fig. 5

From: Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Fig. 5

Synthetic circuit of Mxr1 enhanced scFv expression. After being cultured in BMGY (1% glycerol), cells were cultured in BMMY and induced with 1% methanol daily. a The relative secreted scFv titre is presented. The error bars represent the standard deviation of three biological replicates. One-way ANOVA and the Tukey test were used to determine significance. *, p < 0.05; ***, p < 0.005. b Intracellular proteins after induction were examined by western blotting using an anti-his antibody. GAPDH was used as the loading control

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